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s 100β  (Boster Bio)


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    Structured Review

    Boster Bio s 100β
    S 100β, supplied by Boster Bio, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+100%CE%B2/Anti-S100B%2FS100+Beta+Rabbit+Monoclonal+Antibody/us12599634-160-49-55
    Average 89 stars, based on 4 article reviews
    s 100β - by Bioz Stars, 2026-09
    89/100 stars

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    Related Articles

    Incubation:

    Article Title: Nerve guidance conduit comprising neural crest stem-like cells and/or Schwann cell precursor-like cells and methods of making and using the same
    Article Snippet: Images were captured using Olympus inverted fluorescence microscope (IX73). .. Cryosections (10 μm thickness) prepared from the 3D-collagen hydrogel encapsulated with GMSCs were permeabilized in 0.5% Triton X-100 for 20 min and blocked with 2.5% goat serum in PBS at room temperature for 1 h. Then, the sections were incubated with the following primary antibodies at 4° C. overnight: S-100β (M00979-1; rabbit monoclonal IgG, 1:200; Boster, Pleasanton, Calif.), p75 (AHP1014; rabbit IgG, 1:200; BioRad), BDNF (ab108319; rabbit IgG, 1:200; Abcam), and GDNF (ab18956; rabbit IgG, 1:200; Abcam). .. Following washing twice with PBS, sections were incubated with Alexa FluorR 488 Donkey anti-rabbit IgG (minimal x-reactivity) antibody (406,416; 1:300, BioLegend) at room temperature for 1 h, while an isotype-matched control antibody, FITC Donkey anti-rabbit IgG (minimal x-reactivity) antibody (BioLegend), was used as a negative control.

    Article Title: Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves
    Article Snippet: .. After permeabilization in 0.5%Triton X‐100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4 °C overnight: S-100β (M00979-1; rabbit monoclonal IgG, 1:200; Boster), β-tubulin III (MCA2047; mIgG1, 1:200; BioRad), human nuclei (GTX82624; mIgG, 1:200; GenTex), BDNF (ab108319; rabbit IgG, 1:200; Abcam), GDNF (ab18956; rabbit IgG, 1:200; Abcam), active caspase-3 (AB3623; rabbit IgG, 1:200; Millipore), CD68 (MCA341GA; mouse IgG, 1:200; BioRad), arginase-1 (16,001–1-AP; rabbit IgG, 1:200; Proteintech), iNOS (18,985–1-AP; rabbit IgG, 1:200; Proteintech), and CD206 (18,704–1-AP; rabbit IgG, 1:200; Proteintech). .. After washing twice with PBS, the sections were incubated at room temperature for 1 h with Alexa Fluor ® 488 Donkey anti-rabbit IgG (minimal x-reactivity) antibody (406,416; 1:300, BioLegend) and Alexa Fluor ® 588 goat anti-mouse IgG (minimal x-reactivity) antibody (405,326; 1:300, BioLegend), while corresponding isotype-matched control antibodies (BioLegend) were used as negative controls.

    Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits
    Article Snippet: Collagen hydrogel (40 μl at a final concentration of 4 mg/mL) encapsulated with GMSCs (2 × 10 6 /mL) was filled into the commercially available AxoGuard Nerve protector or connector (2 mm in internal diameter × 10 mm length) made of porcine submucosal ECM (Cook Biotech) and incubated at 37 °C for 20 min for gel formation followed by culturing in complete α-MEM medium for 24 h. Afterwards, the constructs were used for further in vivo studies. .. Cryosections prepared from 3D-collagen gel or GMSC-seeded nerve conduits were blocked and permeabilized for 1 h at room temperature in PBS with 2.5% goat serum and 0.5%Triton X‐100, followed by incubation with the following primary antibodies at the appropriate dilution overnight at 4 °C: p75 (mouse IgG, 1:200, Sigma), SOX-9 (rabbit IgG, 1:200, Cell Signal Tech), SOX-10 (mouse IgG, 1:200, R & D), S-100β (rabbit IgG, 1:200, Boster Biological Tech), NOTCH3 (rabbit IgG, 1:200, Abcam), HES1 (rabbit IgG, 1:200, Cell Signaling Tech), vinculin (mouse IgG, 1:400, Millipore), TRITC-conjugated phalloidin (1:400, Millipore), BDNF (rabbit IgG, 1:200, Abcam), GDNF (rabbit IgG, 1:200, Abcam), or NGF (rabbit IgG, 1:200, Abcam). .. After washing with PBS, cells were incubated with appropriate secondary antibodies at room temperature for 1 h: goat anti-rabbit IgG–AlexaFluo-488 (1:300, BioLegend).

    Article Title: Nerve guidance conduit comprising neural crest stem-like cells and/or Schwann cell precursor-like cells and methods of making and using the same
    Article Snippet: .. After permeabilization in 0.5% Triton X-100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4° C. overnight: S-100β (M00979-1; rabbit monoclonal IgG, 1:200; Boster), β-tubulin III (MCA2047; mIgG1, 1:200; BioRad), human nuclei (GTX82624; mIgG, 1:200; GenTex), BDNF (ab108319; rabbit IgG, 1:200; Abcam), GDNF (ab18956; rabbit IgG, 1:200; Abcam), active caspase-3 (AB3623; rabbit IgG, 1:200; Millipore), CD68 (MCA341GA; mouse IgG, 1:200; BioRad), arginase-1 (16,001-1-AP; rabbit IgG, 1:200; Proteintech), iNOS (18,985-1-AP; rabbit IgG, 1:200; Proteintech), and CD206 (18,704-1-AP; rabbit IgG, 1:200; Proteintech). .. After washing twice with PBS, the sections were incubated at room temperature for 1 h with Alexa FluorR 488 Donkey anti-rabbit IgG (minimal x-reactivity) antibody (406,416; 1:300, BioLegend) and Alexa FluorR 588 goat anti-mouse IgG (minimal x-reactivity) antibody (405,326; 1:300, BioLegend), while corresponding isotype-matched control antibodies (BioLegend) were used as negative controls.

    Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration
    Article Snippet: .. After blocking and permeabilization in PBS with 3% bovine serum albumin (BSA) and 0.5%Triton X‐100 at room temperature for 1 h, the sections were incubated with primary antibodies for β-tubulin III (1:250; BioRad) or S-100β (1:250; Boster Biological Tech.) overnight at 4 °C, followed by incubation with FITC-conjugated secondary antibodies for 1 h at room temperature. .. Isotype-matched control antibodies (BioLegend) were used as negative controls.

    Article Title: Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves
    Article Snippet: .. Cryosections (10 μm thickness) prepared from the 3D-collagen hydrogel encapsulated with GMSCs were permeabilized in 0.5% Triton X‐100 for 20 min and blocked with 2.5% goat serum in PBS at room temperature for 1 h. Then, the sections were incubated with the following primary antibodies at 4 °C overnight: S-100β (M00979-1; rabbit monoclonal IgG, 1:200; Boster, Pleasanton, CA), p75 (AHP1014; rabbit IgG, 1:200; BioRad), BDNF (ab108319; rabbit IgG, 1:200; Abcam), and GDNF (ab18956; rabbit IgG, 1:200; Abcam). .. Following washing twice with PBS, sections were incubated with Alexa Fluor ® 488 Donkey anti-rabbit IgG (minimal x-reactivity) antibody (406,416; 1:300, BioLegend) at room temperature for 1 h, while an isotype-matched control antibody, FITC Donkey anti-rabbit IgG (minimal x-reactivity) antibody (BioLegend), was used as a negative control.

    Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration
    Article Snippet: .. Cultured cells fixed with 4% paraformaldehyde (PFA) or cryosections of GMSC spheroids were blocked and permeabilized for 1 h at room temperature in PBS with 2.5% goat serum and 0.5%Triton X‐100, followed by incubation with the following primary antibodies at the appropriate dilution overnight at 4 °C: Nestin (mouse IgG, 1:250) (EMD Millipore, Burlington, MA, USA), CD29 (mouse IgG, 1:250) (BD Bioscience, San Jose, CA, USA), cleaved caspase-3 (Rabbit IgG, 1:250) (EMD Millipore), type I collagen (rabbit IgG, 1:250) (Rockland Biotech, Limerick, PA, USA), CD73(mouse IgG, 1:250) (BD Bioscience), CD90 (mouse IgG, 1:250) (BD Bioscience), vimentin (rabbit IgG, 1:250) (Boster Biological Tech., Pleasanton, CA, USA), fibronectin (rabbit IgG, 1:200 (Sigma, St. Louis, MO, USA), laminin 1 (rabbit IgG, 1:200) (EMD Millipore), β-tubulin III (mouse IgG, 1:200) (BioRad, Hercules, CA, USA), and S-100β (rabbit IgG, 1:250) (Boster Biological Tech). .. After washing with PBS, cells were incubated with appropriate secondary antibodies at room temperature for 1 h: Alexa Fluor®594 goat anti-rabbit IgG (FITC) (1:250, BioLegend), Alexa Fluor®488 goat anti-mouse IgG (1:250) (BioLegend, San Diego, CA).

    Blocking Assay:

    Article Title: Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves
    Article Snippet: .. After permeabilization in 0.5%Triton X‐100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4 °C overnight: S-100β (M00979-1; rabbit monoclonal IgG, 1:200; Boster), β-tubulin III (MCA2047; mIgG1, 1:200; BioRad), human nuclei (GTX82624; mIgG, 1:200; GenTex), BDNF (ab108319; rabbit IgG, 1:200; Abcam), GDNF (ab18956; rabbit IgG, 1:200; Abcam), active caspase-3 (AB3623; rabbit IgG, 1:200; Millipore), CD68 (MCA341GA; mouse IgG, 1:200; BioRad), arginase-1 (16,001–1-AP; rabbit IgG, 1:200; Proteintech), iNOS (18,985–1-AP; rabbit IgG, 1:200; Proteintech), and CD206 (18,704–1-AP; rabbit IgG, 1:200; Proteintech). .. After washing twice with PBS, the sections were incubated at room temperature for 1 h with Alexa Fluor ® 488 Donkey anti-rabbit IgG (minimal x-reactivity) antibody (406,416; 1:300, BioLegend) and Alexa Fluor ® 588 goat anti-mouse IgG (minimal x-reactivity) antibody (405,326; 1:300, BioLegend), while corresponding isotype-matched control antibodies (BioLegend) were used as negative controls.

    Article Title: Nerve guidance conduit comprising neural crest stem-like cells and/or Schwann cell precursor-like cells and methods of making and using the same
    Article Snippet: .. After permeabilization in 0.5% Triton X-100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4° C. overnight: S-100β (M00979-1; rabbit monoclonal IgG, 1:200; Boster), β-tubulin III (MCA2047; mIgG1, 1:200; BioRad), human nuclei (GTX82624; mIgG, 1:200; GenTex), BDNF (ab108319; rabbit IgG, 1:200; Abcam), GDNF (ab18956; rabbit IgG, 1:200; Abcam), active caspase-3 (AB3623; rabbit IgG, 1:200; Millipore), CD68 (MCA341GA; mouse IgG, 1:200; BioRad), arginase-1 (16,001-1-AP; rabbit IgG, 1:200; Proteintech), iNOS (18,985-1-AP; rabbit IgG, 1:200; Proteintech), and CD206 (18,704-1-AP; rabbit IgG, 1:200; Proteintech). .. After washing twice with PBS, the sections were incubated at room temperature for 1 h with Alexa FluorR 488 Donkey anti-rabbit IgG (minimal x-reactivity) antibody (406,416; 1:300, BioLegend) and Alexa FluorR 588 goat anti-mouse IgG (minimal x-reactivity) antibody (405,326; 1:300, BioLegend), while corresponding isotype-matched control antibodies (BioLegend) were used as negative controls.

    Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration
    Article Snippet: .. After blocking and permeabilization in PBS with 3% bovine serum albumin (BSA) and 0.5%Triton X‐100 at room temperature for 1 h, the sections were incubated with primary antibodies for β-tubulin III (1:250; BioRad) or S-100β (1:250; Boster Biological Tech.) overnight at 4 °C, followed by incubation with FITC-conjugated secondary antibodies for 1 h at room temperature. .. Isotype-matched control antibodies (BioLegend) were used as negative controls.

    Cell Culture:

    Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration
    Article Snippet: .. Cultured cells fixed with 4% paraformaldehyde (PFA) or cryosections of GMSC spheroids were blocked and permeabilized for 1 h at room temperature in PBS with 2.5% goat serum and 0.5%Triton X‐100, followed by incubation with the following primary antibodies at the appropriate dilution overnight at 4 °C: Nestin (mouse IgG, 1:250) (EMD Millipore, Burlington, MA, USA), CD29 (mouse IgG, 1:250) (BD Bioscience, San Jose, CA, USA), cleaved caspase-3 (Rabbit IgG, 1:250) (EMD Millipore), type I collagen (rabbit IgG, 1:250) (Rockland Biotech, Limerick, PA, USA), CD73(mouse IgG, 1:250) (BD Bioscience), CD90 (mouse IgG, 1:250) (BD Bioscience), vimentin (rabbit IgG, 1:250) (Boster Biological Tech., Pleasanton, CA, USA), fibronectin (rabbit IgG, 1:200 (Sigma, St. Louis, MO, USA), laminin 1 (rabbit IgG, 1:200) (EMD Millipore), β-tubulin III (mouse IgG, 1:200) (BioRad, Hercules, CA, USA), and S-100β (rabbit IgG, 1:250) (Boster Biological Tech). .. After washing with PBS, cells were incubated with appropriate secondary antibodies at room temperature for 1 h: Alexa Fluor®594 goat anti-rabbit IgG (FITC) (1:250, BioLegend), Alexa Fluor®488 goat anti-mouse IgG (1:250) (BioLegend, San Diego, CA).

    Staining:

    Article Title: NECL1 coated PLGA as favorable conduits for repair of injured peripheral nerve.
    Article Snippet: a Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China b Collaborative Innovation Center of Guangxi Biological Medicine, Guangxi Medical University, Nanning, China c Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China d Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China e The Medical and Scientific Research Center, Guangxi Medical University, Nanning, China



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    Image Search Results


    ( A ) Scheme to identify EGFP-expressing cell types by fIHC. Neurons and astrocytes were immunolabeled for NeuN and GFAP, respectively. Cells immunostained for Olig2 or Iba1 were identified as oligodendrocytes and microglia. Cells were detected with Hoechest 33342, NucBlue, in the mounting reagent ProLong Glass. ( B ) Representative immunohistochemical images of marmoset cerebral cortex that received injection of AAV2. Two serial slices were presented: one immunolabeled for GFP, NeuN, and GFAP, and another one for GFP, Olig2, and Iba1, as indicated at each panel. Scale bar, 100 μm.

    Journal: bioRxiv

    Article Title: Optimal AAV capsid/promoter combinations to target specific cell types in the common marmoset cerebral cortex

    doi: 10.1101/2024.05.09.593444

    Figure Lengend Snippet: ( A ) Scheme to identify EGFP-expressing cell types by fIHC. Neurons and astrocytes were immunolabeled for NeuN and GFAP, respectively. Cells immunostained for Olig2 or Iba1 were identified as oligodendrocytes and microglia. Cells were detected with Hoechest 33342, NucBlue, in the mounting reagent ProLong Glass. ( B ) Representative immunohistochemical images of marmoset cerebral cortex that received injection of AAV2. Two serial slices were presented: one immunolabeled for GFP, NeuN, and GFAP, and another one for GFP, Olig2, and Iba1, as indicated at each panel. Scale bar, 100 μm.

    Article Snippet: Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87, Nacalai Tesque, Kyoto, Japan), 0.5% Triton X-100, 0.03% NaN3 in 1 x PB): rat monoclonal anti-GFP antibody (1:1,000; 04404-84; Nacalai Tesque, Kyoto, Japan), mouse monoclonal anti-NeuN antibody (1:1,000; MAB377; Merck), rabbit polyclonal anti-GFAP antibody (1:200; GFAP-Rb-Af800; Nittobo Medical, Tokyo, Japan), rabbit polyclonal anti-S-100β antibody (1:200; S100b-Rb-Af1000, Nittobo Medical), mouse monoclonal anti-Olig2 antibody (1:500; MABN50; Merck) and rabbit polyclonal anti-Iba1 antibody (1:500; 019-19741; Fujifilm Wako Chemicals, Tokyo, Japan).

    Techniques: Expressing, Immunolabeling, Immunohistochemical staining, Injection

    ( A ) Fluorescent images of the cortex immunolabeled for EGFP 4 weeks after AAV injection. Scale bar, 100 μm. (B-E) Graphs showing the ratio of GFP (+) neurons ( B ), GFP (+) oligodendrocytes ( C ), GFP (+) astrocytes ( D ), and GFP (+) microglia (E) to total GFP (+) cells. The dotted lines in the graphs are ratios of respective cell types to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and the dots in the graph indicate the respective values for each of the individual marmosets. The asterisks indicate a statistically significant difference between the AAV5 vector and the other capsids. * p < 0.05, ** p < 0.01 by 1-way ANOVA with Tukey’s post hoc test. n.s., not statistically significant.

    Journal: bioRxiv

    Article Title: Optimal AAV capsid/promoter combinations to target specific cell types in the common marmoset cerebral cortex

    doi: 10.1101/2024.05.09.593444

    Figure Lengend Snippet: ( A ) Fluorescent images of the cortex immunolabeled for EGFP 4 weeks after AAV injection. Scale bar, 100 μm. (B-E) Graphs showing the ratio of GFP (+) neurons ( B ), GFP (+) oligodendrocytes ( C ), GFP (+) astrocytes ( D ), and GFP (+) microglia (E) to total GFP (+) cells. The dotted lines in the graphs are ratios of respective cell types to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and the dots in the graph indicate the respective values for each of the individual marmosets. The asterisks indicate a statistically significant difference between the AAV5 vector and the other capsids. * p < 0.05, ** p < 0.01 by 1-way ANOVA with Tukey’s post hoc test. n.s., not statistically significant.

    Article Snippet: Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87, Nacalai Tesque, Kyoto, Japan), 0.5% Triton X-100, 0.03% NaN3 in 1 x PB): rat monoclonal anti-GFP antibody (1:1,000; 04404-84; Nacalai Tesque, Kyoto, Japan), mouse monoclonal anti-NeuN antibody (1:1,000; MAB377; Merck), rabbit polyclonal anti-GFAP antibody (1:200; GFAP-Rb-Af800; Nittobo Medical, Tokyo, Japan), rabbit polyclonal anti-S-100β antibody (1:200; S100b-Rb-Af1000, Nittobo Medical), mouse monoclonal anti-Olig2 antibody (1:500; MABN50; Merck) and rabbit polyclonal anti-Iba1 antibody (1:500; 019-19741; Fujifilm Wako Chemicals, Tokyo, Japan).

    Techniques: Immunolabeling, Injection, Plasmid Preparation

    ( A ) Immunofluorescent EGFP images of the cortex injected with AAV7 expressing EGFP by the CBh, CMV, or CAG promoter. Scale bar, 100 μm. ( B - E ) Graphs showing ratios of respective EGFP-immunolabeled cell types to total EGFP-expressing cells by AAV7 vectors with the CBh, CMV, and CAG promoters. The dotted lines in the graphs are ratios of respective cell types to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and dots in the graph indicate the respective values for each of the individual marmosets. P values obtained using 1-way ANOVA with Tukey’s post hoc test were described in the graphs. n.d., not detected.

    Journal: bioRxiv

    Article Title: Optimal AAV capsid/promoter combinations to target specific cell types in the common marmoset cerebral cortex

    doi: 10.1101/2024.05.09.593444

    Figure Lengend Snippet: ( A ) Immunofluorescent EGFP images of the cortex injected with AAV7 expressing EGFP by the CBh, CMV, or CAG promoter. Scale bar, 100 μm. ( B - E ) Graphs showing ratios of respective EGFP-immunolabeled cell types to total EGFP-expressing cells by AAV7 vectors with the CBh, CMV, and CAG promoters. The dotted lines in the graphs are ratios of respective cell types to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and dots in the graph indicate the respective values for each of the individual marmosets. P values obtained using 1-way ANOVA with Tukey’s post hoc test were described in the graphs. n.d., not detected.

    Article Snippet: Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87, Nacalai Tesque, Kyoto, Japan), 0.5% Triton X-100, 0.03% NaN3 in 1 x PB): rat monoclonal anti-GFP antibody (1:1,000; 04404-84; Nacalai Tesque, Kyoto, Japan), mouse monoclonal anti-NeuN antibody (1:1,000; MAB377; Merck), rabbit polyclonal anti-GFAP antibody (1:200; GFAP-Rb-Af800; Nittobo Medical, Tokyo, Japan), rabbit polyclonal anti-S-100β antibody (1:200; S100b-Rb-Af1000, Nittobo Medical), mouse monoclonal anti-Olig2 antibody (1:500; MABN50; Merck) and rabbit polyclonal anti-Iba1 antibody (1:500; 019-19741; Fujifilm Wako Chemicals, Tokyo, Japan).

    Techniques: Injection, Expressing, Immunolabeling

    ( A ) Schema depicting the AAV genome structure. ( B ) Immunofluorescent EGFP images of the cerebral cortex received injections of respective AAV vectors. Scale bar, 100 μm. ( C ) Representative image immunostained for EGFP alone (left) and merged image for EGFP and GFAP (right) after injection of the AAV2 vector. Scale bar, 100 μm. ( D ) Graph showing the percentage of GFAP-positive astrocytes to total EGFP-expressing cells 4 weeks after injection of the AAV vector as indicated. The dotted line in the graph shows a ratio of oligodendrocytes to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and dots in the graph indicate the respective values for each of the individual marmosets. n.s., not statistically significant by one-way ANOVA with Tukey’s post hoc test.

    Journal: bioRxiv

    Article Title: Optimal AAV capsid/promoter combinations to target specific cell types in the common marmoset cerebral cortex

    doi: 10.1101/2024.05.09.593444

    Figure Lengend Snippet: ( A ) Schema depicting the AAV genome structure. ( B ) Immunofluorescent EGFP images of the cerebral cortex received injections of respective AAV vectors. Scale bar, 100 μm. ( C ) Representative image immunostained for EGFP alone (left) and merged image for EGFP and GFAP (right) after injection of the AAV2 vector. Scale bar, 100 μm. ( D ) Graph showing the percentage of GFAP-positive astrocytes to total EGFP-expressing cells 4 weeks after injection of the AAV vector as indicated. The dotted line in the graph shows a ratio of oligodendrocytes to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and dots in the graph indicate the respective values for each of the individual marmosets. n.s., not statistically significant by one-way ANOVA with Tukey’s post hoc test.

    Article Snippet: Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87, Nacalai Tesque, Kyoto, Japan), 0.5% Triton X-100, 0.03% NaN3 in 1 x PB): rat monoclonal anti-GFP antibody (1:1,000; 04404-84; Nacalai Tesque, Kyoto, Japan), mouse monoclonal anti-NeuN antibody (1:1,000; MAB377; Merck), rabbit polyclonal anti-GFAP antibody (1:200; GFAP-Rb-Af800; Nittobo Medical, Tokyo, Japan), rabbit polyclonal anti-S-100β antibody (1:200; S100b-Rb-Af1000, Nittobo Medical), mouse monoclonal anti-Olig2 antibody (1:500; MABN50; Merck) and rabbit polyclonal anti-Iba1 antibody (1:500; 019-19741; Fujifilm Wako Chemicals, Tokyo, Japan).

    Techniques: Injection, Plasmid Preparation, Expressing

    ( A ) Schema depicting the AAV genome structure. ( B - C ) Immunolabeled fluorescent images of EGFP in the cerebral cortex that received injection of AAV5 ( B ) or AAVrh10 ( C ) vectors expressing EGFP by the mMBP promoter. The middle immunofluorescence images present an overlay of immunolabeling for EGFP and the oligodendrocyte marker Olig2. The bottom images are magnifications of the boxed areas in the center images. Scale bar, 100 μm. ( D - E ) Summary graphs showing the specificity ( D ) and efficiency ( E ) of oligodendrocyte transduction. The dotted line in the graph indicates a ratio of oligodendrocytes to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and dots in the graph indicate the respective values for each of the individual marmosets. Asterisks indicate statistically significant differences between the AAV5 and AAVrh10. ** p < 0.01, *** p < 0.001 by student’s t-test.

    Journal: bioRxiv

    Article Title: Optimal AAV capsid/promoter combinations to target specific cell types in the common marmoset cerebral cortex

    doi: 10.1101/2024.05.09.593444

    Figure Lengend Snippet: ( A ) Schema depicting the AAV genome structure. ( B - C ) Immunolabeled fluorescent images of EGFP in the cerebral cortex that received injection of AAV5 ( B ) or AAVrh10 ( C ) vectors expressing EGFP by the mMBP promoter. The middle immunofluorescence images present an overlay of immunolabeling for EGFP and the oligodendrocyte marker Olig2. The bottom images are magnifications of the boxed areas in the center images. Scale bar, 100 μm. ( D - E ) Summary graphs showing the specificity ( D ) and efficiency ( E ) of oligodendrocyte transduction. The dotted line in the graph indicates a ratio of oligodendrocytes to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and dots in the graph indicate the respective values for each of the individual marmosets. Asterisks indicate statistically significant differences between the AAV5 and AAVrh10. ** p < 0.01, *** p < 0.001 by student’s t-test.

    Article Snippet: Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87, Nacalai Tesque, Kyoto, Japan), 0.5% Triton X-100, 0.03% NaN3 in 1 x PB): rat monoclonal anti-GFP antibody (1:1,000; 04404-84; Nacalai Tesque, Kyoto, Japan), mouse monoclonal anti-NeuN antibody (1:1,000; MAB377; Merck), rabbit polyclonal anti-GFAP antibody (1:200; GFAP-Rb-Af800; Nittobo Medical, Tokyo, Japan), rabbit polyclonal anti-S-100β antibody (1:200; S100b-Rb-Af1000, Nittobo Medical), mouse monoclonal anti-Olig2 antibody (1:500; MABN50; Merck) and rabbit polyclonal anti-Iba1 antibody (1:500; 019-19741; Fujifilm Wako Chemicals, Tokyo, Japan).

    Techniques: Immunolabeling, Injection, Expressing, Immunofluorescence, Marker, Transduction