s 100β (Boster Bio)
Structured Review
S 100β, supplied by Boster Bio, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+100%CE%B2/Anti-S100B%2FS100+Beta+Rabbit+Monoclonal+Antibody/us12599634-160-49-55
Average 89 stars, based on 4 article reviews
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Incubation:Article Title: Nerve guidance conduit comprising neural crest stem-like cells and/or Schwann cell precursor-like cells and methods of making and using the same Article Snippet: Images were captured using Olympus inverted fluorescence microscope (IX73). .. Cryosections (10 μm thickness) prepared from the 3D-collagen hydrogel encapsulated with GMSCs were permeabilized in 0.5% Triton X-100 for 20 min and blocked with 2.5% goat serum in PBS at room temperature for 1 h. Then, the sections were incubated with the following primary antibodies at 4° C. overnight: Article Title: Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves Article Snippet: .. After permeabilization in 0.5%Triton X‐100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4 °C overnight: Article Title: Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits Article Snippet: Collagen hydrogel (40 μl at a final concentration of 4 mg/mL) encapsulated with GMSCs (2 × 10 6 /mL) was filled into the commercially available AxoGuard Nerve protector or connector (2 mm in internal diameter × 10 mm length) made of porcine submucosal ECM (Cook Biotech) and incubated at 37 °C for 20 min for gel formation followed by culturing in complete α-MEM medium for 24 h. Afterwards, the constructs were used for further in vivo studies. .. Cryosections prepared from 3D-collagen gel or GMSC-seeded nerve conduits were blocked and permeabilized for 1 h at room temperature in PBS with 2.5% goat serum and 0.5%Triton X‐100, followed by incubation with the following primary antibodies at the appropriate dilution overnight at 4 °C: p75 (mouse IgG, 1:200, Sigma), SOX-9 (rabbit IgG, 1:200, Cell Signal Tech), SOX-10 (mouse IgG, 1:200, R & D), Article Title: Nerve guidance conduit comprising neural crest stem-like cells and/or Schwann cell precursor-like cells and methods of making and using the same Article Snippet: .. After permeabilization in 0.5% Triton X-100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4° C. overnight: Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration Article Snippet: .. After blocking and permeabilization in PBS with 3% bovine serum albumin (BSA) and 0.5%Triton X‐100 at room temperature for 1 h, the sections were incubated with primary antibodies for β-tubulin III (1:250; BioRad) or Article Title: Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves Article Snippet: .. Cryosections (10 μm thickness) prepared from the 3D-collagen hydrogel encapsulated with GMSCs were permeabilized in 0.5% Triton X‐100 for 20 min and blocked with 2.5% goat serum in PBS at room temperature for 1 h. Then, the sections were incubated with the following primary antibodies at 4 °C overnight: Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration Article Snippet: .. Cultured cells fixed with 4% paraformaldehyde (PFA) or cryosections of GMSC spheroids were blocked and permeabilized for 1 h at room temperature in PBS with 2.5% goat serum and 0.5%Triton X‐100, followed by incubation with the following primary antibodies at the appropriate dilution overnight at 4 °C: Nestin (mouse IgG, 1:250) (EMD Millipore, Burlington, MA, USA), CD29 (mouse IgG, 1:250) (BD Bioscience, San Jose, CA, USA), cleaved caspase-3 (Rabbit IgG, 1:250) (EMD Millipore), type I collagen (rabbit IgG, 1:250) (Rockland Biotech, Limerick, PA, USA), CD73(mouse IgG, 1:250) (BD Bioscience), CD90 (mouse IgG, 1:250) (BD Bioscience), vimentin (rabbit IgG, 1:250) (Boster Biological Tech., Pleasanton, CA, USA), fibronectin (rabbit IgG, 1:200 (Sigma, St. Louis, MO, USA), laminin 1 (rabbit IgG, 1:200) (EMD Millipore), β-tubulin III (mouse IgG, 1:200) (BioRad, Hercules, CA, USA), and Blocking Assay:Article Title: Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves Article Snippet: .. After permeabilization in 0.5%Triton X‐100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4 °C overnight: Article Title: Nerve guidance conduit comprising neural crest stem-like cells and/or Schwann cell precursor-like cells and methods of making and using the same Article Snippet: .. After permeabilization in 0.5% Triton X-100 for 20 min and blocking with 2.5% goat serum in PBS at room temperature for 1 h, the sections were incubated with primary antibodies at 4° C. overnight: Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration Article Snippet: .. After blocking and permeabilization in PBS with 3% bovine serum albumin (BSA) and 0.5%Triton X‐100 at room temperature for 1 h, the sections were incubated with primary antibodies for β-tubulin III (1:250; BioRad) or Cell Culture:Article Title: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration Article Snippet: .. Cultured cells fixed with 4% paraformaldehyde (PFA) or cryosections of GMSC spheroids were blocked and permeabilized for 1 h at room temperature in PBS with 2.5% goat serum and 0.5%Triton X‐100, followed by incubation with the following primary antibodies at the appropriate dilution overnight at 4 °C: Nestin (mouse IgG, 1:250) (EMD Millipore, Burlington, MA, USA), CD29 (mouse IgG, 1:250) (BD Bioscience, San Jose, CA, USA), cleaved caspase-3 (Rabbit IgG, 1:250) (EMD Millipore), type I collagen (rabbit IgG, 1:250) (Rockland Biotech, Limerick, PA, USA), CD73(mouse IgG, 1:250) (BD Bioscience), CD90 (mouse IgG, 1:250) (BD Bioscience), vimentin (rabbit IgG, 1:250) (Boster Biological Tech., Pleasanton, CA, USA), fibronectin (rabbit IgG, 1:200 (Sigma, St. Louis, MO, USA), laminin 1 (rabbit IgG, 1:200) (EMD Millipore), β-tubulin III (mouse IgG, 1:200) (BioRad, Hercules, CA, USA), and Staining:Article Title: NECL1 coated PLGA as favorable conduits for repair of injured peripheral nerve. Article Snippet: a Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China b Collaborative Innovation Center of Guangxi Biological Medicine, Guangxi Medical University, Nanning, China c Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China d Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China e The Medical and Scientific Research Center, Guangxi Medical University, Nanning, China |
